HCM067 Oxford Agar Base
$199
Intended Use Used for the selective isolation and culture of Listeria monocytogenes. Principle and Interpretation Columbia Blood Agar Base provide carbon and nitrogen sources, vitamins and……
Introduction
Intended Use
Used for the selective isolation and culture of Listeria monocytogenes.
Principle and Interpretation
Columbia Blood Agar Base provide carbon and nitrogen sources, vitamins and growth factors，maintains balanced osmotic pressure; Listeria hydrolyzes aesculin and reacts with iron ions to form black 6,7-dihydroxycoumarin; lithium chloride and other antibiotics can inhibit Gram-negative bacteria and most Gram-positive bacteria; agar is the coagulant of the culture medium.
Formulation
| Ingredients | /liter |
| Columbia blood agar base | 39.0 g |
| Aesculin | 1.0 g |
| Ferric ammonium citrate | 0.5 g |
| Lithium chloride | 15.0 g |
| pH7.0±0.2 at 25°C | |
Preparation
Weigh 55.5 g of this product, add 1000 mL of distilled water or deionized water, stir, heat and boil until completely dissolved, divide into Erlenmeyer bottles, sterilize at 121℃ for 15 min. Cool to about 50℃, add 1 bottle of SR0500 supporting reagent A and 1 bottle of B per 100 mL, mix well and set aside.
Quality Control
The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:
| Quality control strains | Growth |
| Listeria monocytogenes ATCC19115 | Gray-green colonies with a black depression in the center and black surrounding |
| Enterococcus faecalis ATCC29212 | – |
| Escherichia coli ATCC25922 | – |
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
