Cell Culture Applications and Their Broad Range of Fields
News 28 3 月, 2025
Cell culture is a vital technique in scientific research, enabling the study of living tissues and cells. With its diverse applications, cell culture plays a key role in advancing fields like biotechnology, medicine, and genetics. Applications of Cell Culture in Research: Intracellular Activities: Study of energy metabolism, DNA transcription, apop…
Factors Affecting the Accuracy of Laboratory Glassware Measurements
News 28 3 月, 2025
The accuracy of laboratory glassware is crucial for precise measurements in scientific experiments. While calibration and improper use are the primary causes of errors, careful handling and adherence to best practices can minimize discrepancies and enhance measurement reliability. 1. Temperature of the Glassware The capacity of glassware changes…
Petri Dish Usage Guidelines: Cleaning, Types & Precautions
News 28 3 月, 2025
A Petri dish is an essential laboratory tool for microbial and cell cultures. It consists of a flat, round base and a lid. Due to its fragile nature, handling it with care is crucial. Proper cleaning, sterilization, and storage are key to maintaining its integrity and ensuring accurate experimental results. How to Clean a Petri Dish? 1. Soaking B…
How to Eliminate Mycoplasma? Prevention and Solutions for Mycoplasma Contamination in Cell Culture
News 28 3 月, 2025
Introduction During cell culture, unwanted contaminants such as fungi and mold can appear unexpectedly. However, the most troublesome intruder is mycoplasma, which silently infiltrates cell cultures and causes severe damage. This article provides a comprehensive guide on what mycoplasma is, its sources, how it harms cells, and the best ways to detect an…
Understanding Serum: Essential Knowledge for Cell Culture
News 19 3 月, 2025
Serum has been an essential component of culture media since the 1950s, providing key nutrients for cell growth. Despite decades of research, its complex composition is still not fully understood. The quality and composition of serum can vary based on animal age, sex, and physiological conditions. Types of Serum Serum is classified into several types…
Columbia Agar: A Vital Tool in Scientific Research
News 12 3 月, 2025
Introduction Columbia Agar is a versatile culture medium widely used in microbiology, clinical diagnostics, and biological research. With its rich nutrient composition, it supports the growth and identification of various microorganisms, making it an essential tool in laboratories worldwide. What is Columbia Agar? Developed in the 1940s at Columbi…
Baird-Parker Agar: A Key Tool in Staphylococcus Detection
News 12 3 月, 2025
Introduction Baird-Parker Agar is a highly selective culture medium used for isolating and identifying Staphylococcus aureus in food safety testing and clinical diagnostics. Its unique formulation allows efficient detection of this pathogenic bacterium, ensuring public health protection. Composition & Working Principle Baird-Parker Agar contai…
Blank Test: Purpose, Procedure, and Key Considerations
News 3 3 月, 2025
1. What is a Blank Test? A blank test is performed without adding the test sample, using only the solvent or diluent in the same volume, under identical experimental conditions. It helps assess systematic errors and background interference that may affect results. 2. Purpose of a Blank Test Evaluate Systematic Errors: Identifies errors from reage…
How to Identify Contaminants in Microbial Cultures
News 3 3 月, 2025
1. Observing Growth Characteristics Color Changes: Unusual color shifts in the culture medium (e.g., red to yellow or cloudy appearance) may indicate contamination. Colony Morphology: Unexpected colonies differing in shape, color, or size from the intended microorganisms suggest contamination. 2. Microscopic Examination Gram Staining: A stai…
How to Pour Culture Media: Steps and Best Practices
News 3 3 月, 2025
1. Temperature Adjustment The ideal pouring temperature for culture media is around 45°C-50°C. A simple way to estimate this is by touching the bottle with both your palm and the back of your hand—if the palm doesn’t feel hot but the back of the hand does, the temperature is likely around 50°C. If the temperature is too high, condensation will form on t…
Difference Between Nutrient Agar (NA) and Plate Count Agar (PCA)
News 26 11 月, 2024
Introduction For those familiar with microbiological testing, colony count testing varies across industries, including food, air, water, and food contact surfaces. While some standards require Plate Count Agar (PCA), others call for Nutrient Agar (NA). So, what’s the difference between these two culture media? Formulations and Key Differences …