HCM070 Enterobacteriaceae Enrichment Broth Mossel
$199
Intended Use For enriching bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products. Principle and Interpretation Pancreatic digest of gelatin pro……
Introduction
Intended Use
For enriching bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products.
Principle and Interpretation
Pancreatic digest of gelatin provide protein, vitamins and amino acids; glucose carbon source; disodium hydrogen phosphate and potassium dihydrogen phosphate as a buffer; ox bile and brilliant green as selective antibacterial agent, inhibiting the growth of non-Enterobacteriaceae.
Formulation
| Ingredients | /liter |
| Pancreatic digest of gelatin | 10g |
| Glucose monohydrate | 5g |
| Dehydrated ox bile | 20g |
| Potassium dihydrogen phosphate | 2g |
| Disodium hydrogen phosphate dihydrate | 8g |
| Brilliant green | 15mg |
| pH7.2±0.2 at 25°C | |
Preparation
Suspend 45g in 1L of distilled water,stir until completely dissolved and dispense 100 mL into test tubes , heat at 100 °C for 30 min in a waterbath or flowing steam.
Quality Control
The following quality control strains were inoculated and cultured at 30-35℃ for 24h-48h. The results are as follows:
| Quality control strains | Inoculum (CFU) | Growth |
| Escherichia coli ATCC8739 | < 100 | Good growth |
| Pseudomonas aeruginosa ATCC9027 | ||
| Staphylococcus aureus ATCC6538 | > 100 | Inhibited |
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
